Consistent detection and quantification of protein post-translational modifications (PTMs) across samplecohortsis a prerequisite for functional analysis of biological processes.
The syntheticpeptideswere spiked in a 13- step dilution series (highestconcentration: 0.002 µg/µl, see Methods) into a background consisting of an extract of the human cell line HEK-293 to enable assessment of the limits of detectability.

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PeptideAtlas is a multi-organism, publicly accessible compendium ofpeptidesidentified in a large set of tandem mass spectrometry proteomics experiments. More... Mass spectrometer output files are collected for human, mouse, yeast, and several other organisms, and searched using the latest search engines and protein sequences. All results of sequence and spectral library searching are ...

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PeptideQuantitation for the Clinical Research LaboratoryPeptidequantitation is rapidly growing in clinical research as scientists are translatingpeptidebiomarkers identified in basic research to clinical research and utility. This quantitation approach measures uniquepeptidesfrom the proteins that are expected to be present in clinical research samples based on prior knowledge. That is ...

The frequency of residual insulin secretion was determined by measurement of nonfasting serum C-peptideconcentrationin919 individuals with type 1 diabetes according to prespecified groups based on age at diagnosis and duration of disease (from 3 to 81 years' duration). Stimulated C-peptide was measured in those with detectable nonfasting values and a group of those with undetectable values ...